New Bulk Reagents & Kits Fast Track Serum/Plasma Workflows with the ProTrap XG Sample Prep Tool
Ready-to-use buffers and a sample prep kit to make your serum/plasma proteomics workflows easier and faster.
Blog
A Winning Combination for Omics
We’ve invited our colleagues from ReSyn Biosciences to introduce themselves on our Blog. Read on to learn more about their MagReSyn products.
Avoiding Peg Contaminants
Mass spec results can be compromised in many ways, PEG is one of those contaminants that can sneak up on you.
You can’t detect what you don’t inject
As we know, the mass spectrometry data can only reflect the fraction of the sample that gets injected.
You can have your SDS and get rid of it too
Even the best mass spectrometer in the world can only detect the portion of the proteome that is successfully extracted and conserved across the sample prep workflow—so it is critical that the extraction buffer and denaturants that are employed are optimal to recover the entire biological proteome.
SDS: I’m not blue that you’re gone!
Sodium dodecyl sulphate (SDS) also known as sodium lauryl sulfate, is recognized as one of the best detergents for solubilizing protein.
Precipitation…it doesn’t take as long as you think
Every proteomicist has their own list of reasons for avoiding solvent-based precipitation. If only we could eliminate these drawbacks.
Precipitation with a pinch of salt
You’ve just spent weeks nurturing or gathering your precious protein samples. By your calculations, there should be just enough material to complete your experiments. The workflow begins; an extraction, a purification step, maybe even some trypsin, then off to the mass spectrometry lab. But when the results finally come back, the data are, shall we say, beneath your expectations. But why?
Bringing the ProTrap XG to life
Knowing that precipitation can easily be used for reliable recovery, I also wanted to make it simple. I envisioned a device where protein solution, containing all their impurities, combined with organic solvent, and with the push of a button, purified protein is separated from the solvent. I assumed we could buy some type of device to do just that but quickly realized no such tool had yet been made. So I made one.