Protein precipitation is a proven technique and is remarkably effective when performed properly. The challenge is in reproducibility- especially in low microgram protein amounts, when a pellet may not be visible. The ProTrap XG eliminates the possibility that you will accidentally disturb your protein pellet and can improve throughput.
Our co-founder, Alan Doucette, and his team at Dalhousie University regularly publish research posters and papers featuring the ProTrap XG. You can view these at any time in our Posters and Publications online library.
Protein samples that are clarified and that can be precipitated using acetone have been successfully processed using the ProTrap. Our team is actively working on sample processing protocols to provide users with complex samples input parameters for successful precipitation. Low molecular weight proteins (<5kDa) tend not to precipitate well. We are currently exploring new strategies to successfully process these smaller proteins.
The ProTrap XG uses protein precipitation, aggregating the protein in a solvent. Anything soluble in the solvent is washed away when the solvent is removed during a centrifugation step. The precipitated protein is retained by the integrated membrane, while solvent-soluble contaminants are flushed through. Our protocols are based on methods published by our Chief Scientist, Alan Doucette and other researchers in peer-reviewed research articles.
A 96 well-plate version of the ProTrap XG is a part of our product pipeline. Contact us for updates regarding product availability and more.
Our co-founder, Alan Doucette, and his team at Dalhousie University regularly publish research posters and papers featuring the ProTrap XG. You can view these at any time in our Posters and Publications online library.
Our first ProTrap XG reagent kit for bottom-up serum/plasma sample prep is about to launch. We have trial kits available at a discounted rate for early adopters. To get your trial kit contact sales@allumiqs.com OR go to our online store.
The ProTrap requires no special storage conditions, whatsoever.
The ProTrap XG is available in packs of 10, 50, or 100, however customized quantities can be made available upon request. Reach out to us at sales@allumiqs.com to discuss options.
Users typically see greater than 95% recovery of proteins, though your recovery may vary depending on the characteristics of the proteins you are precipitating.
The ProTrap XG is capable of removing over 99.8% of a maximum 1% starting sodium dodecyl sulfate (SDS) solution. It can also eliminate or reduce many salts and other buffer components. We are actively researching just what these limits look like for other buffer components, especially detergents.
Our team is currently exploring new cleanup strategies in low molecular weight (<5 kDa) proteins and peptides through precipitation.
The ProTrap XG device is optimized to process 50 micrograms of protein using our base protocols. Our research team has successfully recovered microgram quantities of protein, down to 5 micrograms. If you need to process more than 50 micrograms or less than 5 micrograms of protein please contact us at support@proteform.com.
Protein samples that are clarified and that can be precipitated using acetone have been successfully processed using the ProTrap. Our team is actively working on sample processing protocols to provide users with complex samples input parameters for successful precipitation. Low molecular weight proteins (<5kDa) tend not to precipitate well. We are currently exploring new strategies to successfully process these smaller proteins.
The ProTrap XG uses protein precipitation, aggregating the protein in a solvent. Anything soluble in the solvent is washed away when the solvent is removed during a centrifugation step. The precipitated protein is retained by the integrated membrane, while solvent-soluble contaminants are flushed through. Our protocols are based on methods published by our Chief Scientist, Alan Doucette and other researchers in peer-reviewed research articles.
The ProTrap requires no special storage conditions, whatsoever.
We accept payment via credit card, PayPal, electronic funds transfer or wire transfer.
Yes, the ProTrap is ready to ship anywhere in the world from our headquarters in Halifax, NS.
You can shop online or we accept orders by email at sales@allumiqs.com.
Or if you need a quote we can provide that too, click here to submit a quote request and one of our team members will be in touch within 48 hours.
The ProTrap XG is available in packs of 10, 50, or 100, however customized quantities can be made available upon request. Reach out to us at sales@allumiqs.com to discuss options.