The ProTrap XG uses protein precipitation, aggregating the protein in a solvent. Anything soluble in the solvent is washed away when the solvent is removed during a centrifugation step. The precipitated protein is retained by the integrated membrane, while solvent-soluble contaminants are flushed through. Our protocols are based on methods published by our Chief Scientist, Alan Doucette and other researchers in peer-reviewed research articles.