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Kinetics of acetone precipitation: investigation of whether protein properties influence precipitation rate under various solvent conditions using the ProTrap XG

Jessica L. Nickerson, Alan A. Doucette, Chemistry Department, Dalhousie University

Introduction

Acetone precipitation is a widely used method of concentrating and purifying complex protein samples in top-down and bottom-up proteomic workflows. Our group previously established that the addition of 1-30 mM salt together with 80% acetone facilitates protein recoveries greater than 95%. We later developed a filtration cartridge (ProTrap XG), which automates precipitation.