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Low Abundance Protein Detection after Acetone Precipitation Using the ProTrap XG

Dr. Sara Lahsaee Little, Dr. Victoria Ann Miller
Proteoform Scientific*

Introduction

Sodium dodecyl sulphate is commonly used during protein extraction before mass spectrometry. Efficient removal of the detergent must be achieved to obtain clean data as residual detergent competes with peptides for ionization.

*Proteoform Scientific is now Allumiqs.