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Rapid authentication of fish species using peptide probes isolated in the ProTrap XG

Alan A. Doucette, Jessica L. Nickerson, Katie Halliday, Joshua Turner, Chemistry Department, Dalhousie University

SUMMARY

  • Either 2% SDS, or 80% formic acid, provides optimal extraction of proteins from fish muscle.
  • Rapid (5 min) acetone precipitation in the ProTrap XG isolates fish protein in high purity.
  • Following tryptic digestion, the ProTrap XG SPE cartridge selectively isolates peptide fractions.
  • Rapid MS infusion of fractionated peptides reveals an abundance of species-specific peptides.
  • Protein sequence similarities across related species demands further work to identify makers.