Rapid authentication of fish species using peptide probes isolated in the ProTrap XG

Rapid authentication of fish species using peptide probes isolated in the ProTrap XG

Alan A. Doucette, Jessica L. Nickerson, Katie Halliday, Joshua Turner, Chemistry Department, Dalhousie University

Introduction

• Either 2% SDS, or 80% formic acid, provides optimal extraction of proteins from fish muscle.
• Rapid (5 min) acetone precipitation in the ProTrap XG isolates fish protein in high purity.
• Following tryptic digestion, the ProTrap XG SPE cartridge selectively isolates peptide fractions.
• Rapid MS infusion of fractionated peptides reveals an abundance of species-specific peptides.
• Protein sequence similarities across related species demands further work to identify makers.