USD $307.00 – USD $896.00
MagReSyn Carboxyl is a magnetic polymeric microsphere support designed for covalent immobilization of primary amine-containing ligands through carbodiimide-succinimidyl ester activation and subsequent amide bond formation.
For covalent ligand immobilization via primary amine residues
MagReSyn® Carboxyl is a magnetic polymeric microparticles support designed for covalent immobilization of primary amine-containing ligands through carbodiimide-succinimidyl ester activation and subsequent amide bond formation. Various strategies for the activation of carboxylate particles have been documented, but the most commonly used method for coupling proteins and other primary amine-containing ligands is through activation by EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) in an aqueous reaction. This reaction can be either single-step or a two-step reaction requiring the addition of N-hydroxysuccinimide (NHS) or the more stable sulfo-NHS yielding NHS ester or sulfo NHS ester intermediates.
Please note that coupling of proteins through NHS-EDC activation can be tricky, requires fresh reagents, and is prone to interference from a range of routine buffers used in life sciences. For simpler coupling of your ligand it is recommended to use MagReSyn® Amine with glutaraldehyde or epoxide activation, or activation of MagReSyn® Carboxyl with commercial coupling reagents such as Anteobind™.
Please review the extensive range of application notes and posters to see how MagReSyn® Carboxyl can be used for your sample preparation in the ReSyn Biosciences Method Library.
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Binding capacity: > 20 mg.ml-1 (BSA)
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol
*Allumiqs only distributes MagResyn products in Canada and United States. If you are not located in these regions, please visit the MagResyn website for more information.
2 ml, 5 ml, 10 ml