USD $378.00USD $1,123.00

Magnetic microparticles with chelated Ti4+ metal ions for highly-specific phosphopeptide enrichment

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Description

Protein phosphorylation is one of the most important post-translational modifications and is a critical process in cellular signaling and regulation of cellular networks. Comprehensive analysis of the phosphoproteome is a challenging task due to the transient and sub-stoichiometric nature of phosphorylation sites. High-throughput phosphoproteome analysis by mass spectrometry requires compatible technologies than can specifically enrich phosphopeptides. MagReSyn® Ti-IMAC microparticle have a flexible linker (to reduce steric hindrance) activated with phosphonate groups for Ti4+ chelation. The unique properties of the proprietary ReSyn microparticle technology allows extremely specific, reproducible enrichment of phosphopeptides from complex biological samples/protein digests. The microparticles can be used either alone, or in combination with MagReSyn® TiO2, MagReSyn® Zr-IMAC and/or MagReSyn® ZrO2 to increase phosphoproteome coverage.

The new High Performance (HP) version of our popular titanium IMAC for phosphopeptide enrichment, was externally validated by the Olsen Lab, and the use first published in Nature Communications, by Dorte B Bekker Jensen et al., 2020. The product offers potentially increased recovery and sample coverage, with application to low-quantity peptide input.

Each batch of product for phosphopeptide enrichment is validated for the application using our stringent mass spectrometry based QC procedures to ensure maximum reproducibility. 

Please review the extensive range of application notes and posters to see how MagReSyn® Ti-IMAC HP can be used for your sample preparation in the ReSyn Biosciences Method Library.

IMPORTANT NOTE: This product has a limited shelf-life of 6 months due the instability of the chelated Titanium ions, please consider carefully how much product is required. Zr-IMAC & Zr-IMAC HP offer similar coverage for most sample types, and we would recommend evaluation of these products for low throughput applications.

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol

*Allumiqs only distributes MagResyn products in Canada and United States. If you are not located in these regions, please visit the MagResyn website for more information.

Additional information

Sizes Available

2 ml, 5 ml, 10 ml