{"id":1754,"date":"2021-12-21T14:23:00","date_gmt":"2021-12-21T18:23:00","guid":{"rendered":"https:\/\/allumiqs.com\/?post_type=science&#038;p=1754"},"modified":"2023-10-02T21:35:18","modified_gmt":"2023-10-03T00:35:18","slug":"acetone-precipitation-effectively-removes-sds-and-results-in-the-detection-of-low-abundance-proteins","status":"publish","type":"science","link":"https:\/\/allumiqs.com\/science\/acetone-precipitation-effectively-removes-sds-and-results-in-the-detection-of-low-abundance-proteins\/","title":{"rendered":"Acetone Precipitation Effectively Removes SDS and Results in the Detection of Low Abundance Proteins"},"content":{"rendered":"\n<p class=\"is-style-default\">Sara Lahsaee Little, Victoria A Miller  Proteoform Scientific Inc.*, Halifax, NS Canada<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Introduction<\/h3>\n\n\n\n<p class=\"is-style-default\">Sodium dodecyl sulphate is a commonly used detergent for protein extraction, particularly for membrane proteins. Adequate removal of the anionic detergent, to levels below 100 ppm, or preferably lower, has been a source of frustration for mass spectrometry users for some time. Precipitation has been used in the past but has a reputation for inconsistent performance. Other methods have been suggested including dialysis and buffer exchange, such as FASP (centrifugal-aided), as well as ion exchange, electrophoresis, and detergent affinity matrices. Many of these strategies have drawbacks including loss of proteins, incomplete detergent removal, and a high cost per use. Standardization of the precipitation process can result in the minimization of sample loss and improvement in reproducibility.<\/p>\n\n\n\n<p>*Proteoform Scientific is now Allumiqs.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/allumiqs.com\/wp-content\/uploads\/2021\/12\/Proteoform-Scientific_ASMS-2021-Poster_Miller-Lahsaee.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download Poster<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Presented at ASMS 2021<\/p>\n","protected":false},"author":2,"featured_media":4772,"template":"","meta":{"inline_featured_image":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false,"download_url":"","footnotes":""},"tags":[],"sciencecat":[24],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science\/1754"}],"collection":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science"}],"about":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/types\/science"}],"author":[{"embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":2,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science\/1754\/revisions"}],"predecessor-version":[{"id":4774,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science\/1754\/revisions\/4774"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/media\/4772"}],"wp:attachment":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/media?parent=1754"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/tags?post=1754"},{"taxonomy":"sciencecat","embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/sciencecat?post=1754"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}