{"id":438,"date":"2018-05-19T21:29:00","date_gmt":"2018-05-20T00:29:00","guid":{"rendered":"http:\/\/stagingproteoform.0442f41.netsolhost.com\/?post_type=science&#038;p=438"},"modified":"2023-10-03T11:48:16","modified_gmt":"2023-10-03T14:48:16","slug":"membrane-based-sds-depletion-ahead-of-peptide-and-protein-analysis","status":"publish","type":"science","link":"https:\/\/allumiqs.com\/science\/membrane-based-sds-depletion-ahead-of-peptide-and-protein-analysis\/","title":{"rendered":"Membrane-based SDS depletion ahead of peptide and protein analysis"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">Membrane-based SDS depletion ahead of peptide and protein analysis by mass spectrometry<\/h4>\n\n\n\n<p>Nicole Unterlander, Alan A. Doucette<br>Department of Chemistry, Dalhousie University<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Introduction<\/h4>\n\n\n\n<p>SDS interferes with both bottom-up and top-down MS analysis and must be removed prior to detection1. For bottom-up proteomics (BUP), a popular depletion method is FASP, however sample losses can often be 50% or greater2,3. Acetone precipitation is favoured for top-down proteomics (TDP), which we have shown can be done inside a centrifugal membrane filter cartridge to enhance reproducibility4. Our group recently introduced an electrophoretic purification method called transmembrane  electrophoresis (TME) as an alternative depletion strategy for both BUP and TDP studies (Figure 1)5. Here we benchmark the effectiveness of TME as compared to these two popular SDS-depletion methods<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/allumiqs.com\/wp-content\/uploads\/2020\/06\/CNPN2018_poster.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download Poster<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Presented at Canadian National Proteomics Network (CNPN) Annual Symposium 2018<\/p>\n","protected":false},"author":2,"featured_media":4827,"template":"","meta":{"inline_featured_image":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false,"download_url":"","footnotes":""},"tags":[],"sciencecat":[24],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science\/438"}],"collection":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science"}],"about":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/types\/science"}],"author":[{"embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":2,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science\/438\/revisions"}],"predecessor-version":[{"id":4829,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/science\/438\/revisions\/4829"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/media\/4827"}],"wp:attachment":[{"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/media?parent=438"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/tags?post=438"},{"taxonomy":"sciencecat","embeddable":true,"href":"https:\/\/allumiqs.com\/wp-json\/wp\/v2\/sciencecat?post=438"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}