What type of yield can I expect with the ProTrap XG?
Users typically see greater than 95% recovery of proteins, though your recovery may vary depending on the characteristics of the proteins you are precipitating.
FAQ Category: Sample Prep
What level of purity can I expect with the ProTrap XG?
The ProTrap XG is capable of removing over 99.8% of a maximum 1% starting sodium dodecyl sulfate (SDS) solution. It can also eliminate or reduce many salts and other buffer components. We are actively researching just what these limits look like for other buffer components, especially detergents.
Does the ProTrap XG do peptide cleanup?
Our team is currently exploring new cleanup strategies in low molecular weight (<5 kDa) proteins and peptides through precipitation.
How much protein can the ProTrap XG prepare?
The ProTrap XG device is optimized to process 50 micrograms of protein using our base protocols. Our research team has successfully recovered microgram quantities of protein, down to 5 micrograms. If you need to process more than 50 micrograms or less than 5 micrograms of protein please contact us at support@proteform.com.
What type of samples can be used in the ProTrap XG?
Protein samples that are clarified and that can be precipitated using acetone have been successfully processed using the ProTrap. Our team is actively working on sample processing protocols to provide users with complex samples input parameters for successful precipitation. Low molecular weight proteins (<5kDa) tend not to precipitate well. We are currently exploring new strategies to successfully process these smaller proteins.
How does the ProTrap XG clean samples?
The ProTrap XG uses protein precipitation, aggregating the protein in a solvent. Anything soluble in the solvent is washed away when the solvent is removed during a centrifugation step. The precipitated protein is retained by the integrated membrane, while solvent-soluble contaminants are flushed through. Our protocols are based on methods published by our Chief Scientist, Alan Doucette and other researchers in peer-reviewed research articles.