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Evaluating the reproducibility of bottom-up proteome sample
preparation in the ProTrap XG through quantitative mass spectrometry

Jessica L. Nickerson*1, Alan A. Doucette1, Peter D. Wentzell1, Hugo Gagnon2

1Department of Chemistry, Dalhousie University, Halifax, Canada 1PhenoSwitch Bioscience*, Sherbrooke, Canada


With advances in LC-MS instrumentation, the precision of quantitative proteomics is increasing. However, sample preparation strategies remain a significant source of variance particularly due to biases in recovery and variable digestion completion.

*PhenoSwitch Bioscience is now Allumiqs.