Evaluating the reproducibility of bottom-up proteome sample
preparation in the ProTrap XG through quantitative mass spectrometry

Jessica L. Nickerson*¹, Alan A. Doucette¹, Peter D. Wentzell¹, Hugo Gagnon²

¹Department of Chemistry, Dalhousie University, Halifax, Canada ¹PhenoSwitch Bioscience, Sherbrooke, Canada

Introduction

With advances in LC-MS instrumentation, the precision of quantitative proteomics is increasing. However, sample preparation strategies remain a significant source of variance particularly due to biases in recovery and variable digestion completion.